Articles

The list below contains peer-reviewed journal articles to which we contributed.

2023

229 Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles (pdf, 6 MB)
Sean M. Davidson, Chantal M. Boulanger, Elena Aikawa, Lina Badimon, Lucio Barile, Christoph J. Binder, Alain Brisson, Edit Buzas, Costanza Emanueli, Felix Jansen, Miroslava Katsur, Romaric Lacroix, Sai Kiang Lim, Nigel Mackman, Manuel Mayr, Philippe Menasché, Rienk Nieuwland, Susmita Sahoo, Kaloyan Takov, Thomas Thum, Pieter Vader, Marca H.M. Wauben, Kenneth Witwer, and Joost P.G. Sluijter
Cardiovascular Research 119 (1), 45-63 (2023)

2022

228 Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry (pdf, 1 MB)
Angela A.F. Gankema, Bo Li, Rienk Nieuwland, and Edwin van der Pol
Cytometry Part A 101 (12), 1049-56 (2022)
227 Shock-driven endotheliopathy in trauma patients is associated with leucocyte derived extracellular vesicles (pdf, 1 MB)
Romein, W.G. Dujardin, Jeske E.C. Kisters, Mathijs R. Wirtz, Najat Hajji, Anita M. Tuip-de Boer, Jakob Stensballe, Pär I. Johansson, Karim Brohi, Ross A. Davenport, Christine Gaarder, Simon Stanworth, Marc Maegele, Rienk Nieuwland, Edwin van der Pol, and Nicole P. Juffermans
International Journal of Molecular Sciences 23 (24), 15990 (2022)
226 Rapid and efficient isolation platform for plasma extracellular vesicles: EV-FISHER (pdf, 2 MB)
Wei-Lun Pan, Jun-Jie Feng, Ting-Ting Luo, Yong Tan, Bo Situ, Rienk Nieuwland, Jing-Yun Guo, Chun-Chen Liu, Han Zhang, Jing Chen, Wen-Hua Zhang, Jun Chen, Xian-Hua Chen, Hong-Yue Chen, Lei Zheng, Jin-Xiang Chen, and Bo Li
Journal of Extracellular Vesicles 11 (11), e12281 (2022)
225 Removal of platelets from blood plasma to improve the quality of extracellular vesicle research (pdf, 1 MB)
Britta Bettin, Aleksandra Gasecka, Bo Li, Bert Dhondt, An Hendrix, Rienk Nieuwland, and Edwin van der Pol
Journal of Thrombosis and Haemostasis 20 (11), 2679-85 (2022)
224 Extracellular vesicles from amniotic fluid, milk, saliva, and urine expose complexes of tissue factor and activated factor VII (pdf, 3 MB)
Yong Hu, Andreas Repa, Ton Lisman, Guelen Yerlikaya-Schatten, Chi Hau, Ingrid Pabinger, Cihan Ay, Rienk Nieuwland, and Johannes Thaler
Journal of Thrombosis and Haemostasis 20 (10), 2306-2312 (2022)
223 Coagulation signaling from amniotic fluid to fetal skin (pdf, 1 MB)
Yong Hu, Anke Scharrer, Chi Hau, Cihan Ay, René J. Berckmans, Wolfram Ruf, Rienk Niuewland, and Johannes Thaler
Blood Advances 6 (19), 5538-5541 (2022)
222 Method to traceably determine the refractive index by measuring the angle of minimum deviation (pdf, 1 MB)
Martine Kuiper, Richard Koops, Rienk Nieuwland, and Edwin van der Pol
Metrologia 59 (5), 055006 (2022)
221 EDTA stabilizes the concentration of platelet-derived extracellular vesicles during blood collection and handling (pdf, <1 MB)
Naomi C. Buntsma, Aleksandra Gąsecka, Yvo B.W.E.M. Roos, Ton G. Leeuwen, Edwin, van der Pol, and Rienk Nieuwland
Platelets 33 (5), 764-71 (2022)
220 An imaging flow cytometry-based methodology for the analysis of single extracellular vesicles in unprocessed human plasma (pdf, 3 MB)
Wouter W. Woud, Edwin van der Pol, Erik Mul, Martin J. Hoogduijn, Carla C. Baan, Karin, Boer, and Ana Merino
Communications Biology 5 (633), 1-4 (2022)
219 Reproducibility of extracellular vesicle research (pdf, 1 MB)
Rienk Nieuwland, Pia R.M. Siljander, Juan M. Falcón-Pérez, and Kenneth W. Witwer
European Journal of Cell Biology 101 (3), 151226 (2022)
218 Calibration and standardization of extracellular vesicle measurements by flow cytometry for translational prostate cancer research (pdf, 4 MB)
Yohan Kim, Edwin van der Pol, Ali Arafi, Ishwor Thapa, Cameron Britton, Jorgena Kosti, Siyang Song, Vidhu B. Joshi, Ree Erickson, Hesham Ali, and Fabrice Lucien
Nanoscale 14, 9781-95 (2022)
217 Diagnostic performance of circulating miRNAs and extracellular vesicles in acute ischemic stroke (pdf, 2 MB)
Ceren Eyileten, Daniel Jakubik, Andleeb Shahzadi, Aleksandra Gąsecka, Edwin van der Pol, Salvatore De Rosa, Dominika Siwik, Magdalena Gajewska, Dagmara Mirowska-Guzel, Iwona Kurkowska-Jastrzębska, Anna Czlonkowska, and Marek Postula
International Journal of Molecular Sciences 23, 4530 (2022)
216 Intraperitoneal activation of coagulation and fibrinolysis in patients with cirrhosis and ascites (pdf, <1 MB)
Johannes Thaler, Ton Lisman, Peter Quehenberger, Lena Hell, Philipp Schwabl, Bernhard Scheiner, Theresa Bucsics, Rienk Nieuwland, Cihan Ay, Michael Trauner, Ingrid Pabinger, Thomas Reiberger, and Mattias Mandorfer
Thrombosis and Haemostasis 122 (03), 353-362 (2022)
215 Processing methods of donor human milk evaluated by a blood plasma clotting assay (pdf, 1 MB)
Yong Hu, Eva Kontopodi, Elise Mank, Chris H.P. van den Akker, Johannes B. van Goudoever, Kasper Hettinga, Ruurd M. van Elburg, Johannes Thaler, and Rienk Nieuwland
Innovative Food Science & Emerging Technologies 76, 102938 (2022)
214 Minimum information to report about a flow cytometry experiment on extracellular vesicles: communication from the ISTH SSC Subcommittee on Vascular Biology (pdf, 1 MB)
Edwin van der Pol, Joshua A. Welsh, and Rienk Nieuwland
Journal of Thrombosis and Haemostasis 20 (1), 245-51 (2022)

2021

213 Misinterpretation of solid sphere equivalent refractive index measurements and smallest detectable diameters of extracellular vesicles by flow cytometry (pdf, 1 MB)
Edwin van der Pol, Ton G. van Leeuwen, and Xiaomei Yan
Scientific Reports 11, 24151 (2021)
212 Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles (pdf, 1 MB)
Kenneth W. Witwer, Deborah CI Goberdhan, Lorraine O'Driscoll, Clotilde Théry, Joshua A. Welsh, Cherie Blenkiron, Edit I. Buzás, Dolores Di Vizio, Uta Erdbrügger, Juan M. Falcón-Pérez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Jan Lötvall, Rienk Nieuwland, Takahiro Ochiya, Sophie Rome, Susmita Sahoo, and Lei Zheng
Journal of Extracellular Vesicles 10 (14), e12182 (2021)
211 Extracellular Vesicles in Human Milk (pdf, 2 MB)
Yong Hu, Johannes Thaler, and Rienk Nieuwland
Pharmaceuticals 14 (10), 1050 (2021)
210 Platelets are hyperactivated but show reduced glycoprotein VI reactivity in COVID-19 patients (pdf, <1 MB)
Valentine Léopold, Liza Pereverzeva, Alex R. Schuurman, Tom DY Reijnders, Anno Saris, Justin de Brabander, Christine CA van Linge, Renée A. Douma, Osoul Chouchane, Rienk Nieuwland, Joost W. Wiersinga, Cornelis van 't Veer, and Tom van der Poll
Thrombosis and Haemostasis 121 (09), 1258-1262 (2021)
209 Mesenchymal stem cell-derived extracellular vesicles conditionally ameliorate bone marrow failure symptoms in an immune-mediated aplastic anemia mouse model (pdf, 2 MB)
Mohammad A. Gholampour, Saeid Abroun, Rienk Nieuwland, Seyed J. Mowla, and Sara Soudi
Journal of Cellular Physiology 236 (8), 6055-6067 (2021)
208 Estradiol-driven metabolism in transwomen associates with reduced circulating extracellular vesicle microRNA-224/452 (pdf, 8 MB)
Barend W. Florijn, Jacques M.G.J. Duijs, Maartje Klaver, Eline N. Kuipers, Sander Kooijman, Jurrien Prins, Huayu Zhang, Hetty C.M. Sips, Wendy Stam, Maaike Hanegraaf, Ronald W.A.L. Limpens, Rienk Nieuwland, Bas B. van Rijn, Ton J. Rabelink, Patrick C.N. Rensen, Martin den Heijer, Roel Bijkerk, and Anton Jan van Zonneveld
European Journal of Endocrinology 185 (4), 539-552 (2021)
207 Quantification of light scattering detection efficiency and background in flow cytometry (pdf, 1 MB)
Leonie de Rond, Frank A.W. Coumans, Joshua A. Welsh, Rienk Nieuwland, Ton G. Leeuwen, and Edwin van der Pol
Cytometry Part A 99 (7), 671-9 (2021)
206 ADAM9-Responsive mesoporous silica nanoparticles for targeted drug delivery in pancreatic cancer (pdf, 10 MB)
E.J. Slapak, L. Kong, M. El Mandili, R. Nieuwland, A. Kros, M.F. Bijlsma, and C.A. Spek
Cancers 13 (13), 3321 (2021)
205 Novel mechanism regulating tissue factor activity (pdf, 1 MB)
Rienk Nieuwland
Blood 138 (4), 289-291 (2021)
204 Platelet removal by single-step centrifugation (pdf, 1 MB)
Linda G. Rikkert, Frank AW Coumans, Chi M. Hau, Leon WMM Terstappen, and Rienk Nieuwland
Platelets 32 (4), 440-443 (2021)
203 MIFlowCyt-EV: The next chapter in the reporting and reliability of single extracellular vesicle flow cytometry experiments (pdf, 1 MB)
Joshua A. Welsh, Vera A., Tang, Edwin van der Pol, and André Görgens
Cytometry Part A 99 (4), 365-8 (2021)
202 Secreted therapeutics: monitoring durability of microRNA-based gene therapies in the central nervous system (pdf, 2 MB)
Marina Sogorb-Gonzalez, Carlos Vendrell-Tornero, Jolanda Snapper, Anouk Stam, Sonay Keskin, Jana Miniarikova, Elisabeth A. Spronck, Martin de Haan, Rienk Nieuwland, Pavlina Konstantinova, Sander J. van Deventer, Melvin M. Evers, and Astrid Vallès
Brain Communications 3 (2), fcab054 (2021)
201 Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing (pdf, 3 MB)
Michael Cimorelli, Rienk Nieuwland, Zoltán Varga, and Edwin van der Pol
Plos One 16 (4), e0249603 (2021)
200 Prostacyclin analogues inhibit platelet reactivity, extracellular vesicle release and thrombus formation in patients with pulmonary arterial hypertension (pdf, 1 MB)
Aleksandra Gąsecka, Marta Banaszkiewicz, Rienk Nieuwland, Edwin van der Pol, Najat Hajji, Hubert Mutwil, Sylwester Rogula, Wiktoria Rutkowska, Kina Pluta, Ceren Eyileten, Marek Postula, Szymon Darocha, Zenon Huczek, Grzegorz Opolski, Krzysztof J. Filipiak, Adam Torbicki, and Marcin Kurzyna
Journal of Clinical Medicine 10, 1024 (2021)
199 Reliable measurements of extracellular vesicles by clinical flow cytometry (pdf, 1 MB)
Martine Kuiper, Arthur van de Nes, Rienk Nieuwland, Zoltan Varga, and Edwin van der Pol
American Journal of Reproductive Immunology 85 (2), e13350 (2021)
198 Cellular origin and microRNA profiles of circulating extracellular vesicles in different stages of diabetic nephropathy (pdf, 1 MB)
Melissa Uil, Chi M. Hau, Mohamed Ahdi, James D. Mills, Jesper Kers, Moin A. Saleem, Sandrine Florquin, Victor E.A. Gerdes, Rienk Nieuwland, and Joris J.T.H. Roelofs
Clinical Kidney Journal 14 (1), 358-365 (2021)
197 Plasma concentrations of extracellular vesicles are decreased in patients with post-infarct cardiac remodelling (pdf, 1 MB)
Aleksandra Gąsecka, Kinga Pluta, Katarzyna Solarska, Bartłomiej Rydz, Ceren Eyileten, Marek Postuła, Edwin van der Pol, Rienk Nieuwland, Monika Budnik, Janusz Kochanowski, Miłosz Jaguszewski, Łukasz Szarpak, Tomasz Mazurek, Agnieszka Kapłon-Cieślicka, Grzegorz Opolski, and Krzysztof J. Filipiak
Biology 10 (2), 97 (2021)

2020

196 Rigor and standardization of extracellular vesicle research: Paving the road towards robustness (pdf, <1 MB)
Rienk Nieuwland, Juan M. Falcón-Pérez, Clotilde Théry, and Kenneth W. Witwer
Journal of Extracellular Vesicles 10 (2), e12037 (2020)
195 Human milk triggers coagulation via tissue factor-exposing extracellular vesicles (pdf, 2 MB)
Yong Hu, Lena Hell, Ruth Anna Kendlbacher, Najat Hajji, Chi Hau, Annemieke van Dam, René J. Berckmans, Lukas Wisgrill, Cihan Ay, Ingrid Pabinger, A. Brisson, A. Repa, R. Nieuwland, and J. Thaler
Blood Advances 4 (24), 6274-82 (2020)
194 Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size, and concentration (pdf, 8 MB)
Joshua A. Welsh, Edwin van der Pol, Britta A. Bettin, David R.F. Carter, An Hendrix, Metka Lenassi, Marc-André Langlois, Alicia Llorente, Arthur S. van de Nes, Rienk Nieuwland, Vera Tang, Lili Wang, Kenneth W. Witwer, and Jennifer C. Jones
Journal of Extracellular Vesicles 9, 1816641 (2020)
193 International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells: considerations for potential therapeutic agents to suppress coronavirus disease-19 (pdf, <1 MB)
Verena Börger, Daniel J. Weiss, Johnathon D. Anderson, Francesc E. Borràs, Benedetta Bussolati, David R.F. Carter, Massimo Dominici, Juan M. Falcón-Pérez, Mario Gimona, Andrew F. Hill, Andrew M. Hoffman, Dominique de Kleijn, Bruce L. Levine, Rebecca Lim, Jan Lötvall, S. Alex Mitsialis, Marta Monguió-Tortajada, Maurizio Muraca, Rienk Nieuwland, Anna Nowocin, Lorraine O'Driscoll, Luis A. Ortiz, Donald G. Phinney, Ilona Reischl, Eva Rohde et al.
Cytotherapy 22 (9), 482-485 (2020)
192 Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization subcommittee (pdf, 1 MB)
Felix Royo, Clotilde Théry, Juan M. Falcón-Pérez, Rienk Nieuwland, and Kenneth W. Witwer
Cells 9 (9), 1955 (2020)
191 Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery (pdf, 1 MB)
Mari Palviainen, Mayank Saraswat, Zoltan Varga, Diana Kitka, Maarit Neuvonen, Maija Puhka, Sakari Joenväärä, Risto Renkonen, Rienk Nieuwland, Maarit Takatalo, and Pia R.M. Siljander
Plos One 15 (8), e0236439 (2020)
190 Urinary mitochondrial DNA associates with delayed graft function following renal transplantation (pdf, 1 MB)
Marcel P.B. Jansen, Wilco P.C. Pulskens, Melissa Uil, Nike Claessen, Gerrie Nieuwenhuizen, Dorien Standaar, Chi M. Hau, Rienk Nieuwland, Sandrine Florquin, Frederike J. Bemelman, Jaklien C. Leemans, and Joris J.T.H. Roelofs
Nephrology Dialysis Transplantation 35 (8), 1320-1327 (2020)
189 Role of P2Y receptors in platelet extracellular vesicle release (pdf, 2 MB)
Aleksandra Gasecka, Sylwester Rogula, Ceren Eyileten, Marek Postuła, Miłosz J. Jaguszewski, Janusz Kochman, Tomasz Mazurek, Rienk Nieuwland, and Krzysztof J. Filipiak
International Journal of Molecular Sciences 21 (17), 6065 (2020)
188 Rate zonal centrifugation can partially separate platelets from platelet-derived vesicles (pdf, 1 MB)
Linda G. Rikkert, Mendel Engelaer, Chi M. Hau, Leon W.M.M. Terstappen, Rienk Nieuwland, and Frank A.W. Coumans
Research and Practice in Thrombosis and Haemostasis 4 (6), e12366 (2020)
187 Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host (pdf, 2 MB)
Michiel L. Bexkens, Renske A. van Gestel, Bas van Breukelen, Rolf T. Urbanus, Jos F. Brouwers, Rienk Nieuwland, Aloysius G.M. Tielens, and Jaap J. van Hellemond
Molecular and Biochemical Parasitology 238, 111296 (2020)
186 A systematic approach to improve scatter sensitivity of a flow cytometer for detection of extracellular vesicles (pdf, 2 MB)
Leonie de Rond, Edwin van der Pol, Paul R. Bloemen, Tina van den Broeck, Ludo Monheim, Rienk Nieuwland, Ton G. van Leeuwen, and Frank A.W. Coumans
Cytometry Part A 97 (6), 582-91 (2020)
185 Detection of extracellular vesicles in plasma and urine of prostate cancer patients by flow cytometry and surface plasmon resonance imaging (pdf, 1 MB)
Linda G. Rikkert, Leonie De Rond, Annemieke van Dam, Ton G. van Leeuwen, Frank A.W. Coumans, Theo M. de Reijke, Leon W.M.M. Terstappen, and Rienk Nieuwland
Plos One 15 (6), e0233443 (2020)
184 Poor perfusion of the microvasculature in peritoneal metastases of ovarian cancer (pdf, 2 MB)
Arnoud W. Kastelein, Laura M.C. Vos, Juliette O.A.M. van Baal, Jasper J. Koning, Vashendriya V.V. Hira, Rienk Nieuwland, Willemien J. van Driel, Zühre Uz, Thomas M. van Gulik, Jacco van Rheenen, Can Ince, Jan-Paul W.R. Roovers, Cornelis J.F. van Noorden, and Christianne A.R. Lok
Clinical & Experimental Metastasis 37 (2), 293-304 (2020)
183 Cancer-ID: towards identification of cancer by tumor-derived extracellular vesicles in blood (pdf, 2 MB)
Linda G. Rikkert, Pepijn Beekman, Jaap Caro, Frank A.W. Coumans, Agustin Enciso Martinez, Guido Jenster, Séverine Le Gac, Wooje Lee, Ton G. van Leeuwen, Gyllion B. Loozen, Afroditi Nanou, Rienk Nieuwland, Herman L. Offerhaus, Cees Otto, D. Michiel Pegtel, Melissa C. Piontek, Edwin van der Pol, Leonie de Rond, Wouter H. Roos, Richard B.M. Schasfoort, Marca H.M. Wauben, Han Zuilhof, and Léon W.M.M. Terstappen
Frontiers in Oncology 10, 608 (2020)
182 MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments (pdf, 3 MB)
Joshua A. Welsh, Edwin van der Pol, Ger J.A. Arkesteijn, Michel Bremer, Alain Brisson, Frank Coumans, Françoise Dignat-George, Erika Duggan, Ionita Ghiran, Bernd Giebel, André Görgens, An Hendrix, Romaric Lacroix, Joanne Lannigan, Sten F.W.M. Libregts, Estefanía Lozano-Andrés, Aizea Morales-Kastresana, Stephane Robert, Leonie de Rond, Tobias Tertel, John Tigges, Olivier de Wever, Xiaomei Yan, Rienk Nieuwland, Marca H.M. Wauben et al.
Journal of Extracellular Vesicles 9, 1713526 (2020)
181 Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles (pdf, 1 MB)
Agustin Enciso-Martinez, Edwin van der Pol, Aufried T.M. Lenferink, Leon W.M.M. Terstappen, Ton G. van Leeuwen, and Cees Otto
Nanomedicine: Nanotechnology, Biology and Medicine 24, 102109 (2020)
180 Label-free identification and chemical characterisation of single extracellular vesicles and lipoproteins by synchronous Rayleigh and Raman scattering (pdf, 3 MB)
Agustin Enciso-Martinez, Edwin van der Pol, Chi M. Hau, Rienk Nieuwland, Ton G. van Leeuwen, Leon W.M.M. Terstappen, and Cees Otto
Journal of Extracellular Vesicles 9, 1730134 (2020)