Articles

The list below contains peer-reviewed journal articles to which we contributed.

2026

276 Automated cryo-EM and supervised machine learning enable reproducible characterization of extracellular vesicles and co-isolating particles
Agustin Enciso-Martinez, Frank G.A. Faas, Anja W.M. de Jong, Ton van Leeuwen, Rienk Nieuwland, Edwin van der Pol, Peter ten Dijke, and Roman I. Koning
Journal of Extracellular Vesicles, in press.
275 Traceable refractive index measurements of liquids to standardize extracellular vesicle flow cytometry
Martine Kuiper, Richard Koops, Rienk Nieuwland, Ton G. van Leeuwen, and Edwin van der Pol
Cytometry Part A, in press.
274 Refractive index of milk fat globules and extracellular vesicles in human milk (pdf, 6 MB)
Johanna R. de Wolf, Edwin van der Pol, Chi M. Hau, Rienk Nieuwland, and Nienke Bosschaart
Biophotonics Discovery 3 (1), 012104 (2026)
273 Particle size distribution and concentration of Intralipid® 20% (pdf, 4 MB)
Mona Shahsavari, Martine Kuiper, Mendel Engelaer, Martin Poinsinet de Sivry-Houle, Ton G. van Leeuwen, and Edwin van der Pol
Journal of Biomedical Optics 31 (1), 015001 (2026)
272 Red blood cell-derived transglutaminase-2 influences thrombus formation (pdf, 20 MB)
Naoual Ouazzani Chahdi, Judith J. de Vries, Hande Eyisoylu, Hanke L. Matlung, Chi Hau, Rienk Nieuwland, Moniek P.M. de Maat, Joost C.M. Meijers, and Robin van Bruggen
Research and Practice in Thrombosis and Haemostasis 10 (1), 103274 (2026)

2025

271 Formation and characterization of stable antifouling colloidal particles via surface-initiated photoinduced electron-transfer reversible addition-fragmentation chain-transfer polymerization for standardized biophysical measurements (external)
Jelle B.J. van den Beukel, Barend van Lagen, Han Zuilhof, and Edwin van der Pol
Langmuir 41 (50), 33751-60 (2025)
270 Calibration of flow cytometers enables reproducible measurements of extracellular vesicle concentrations and reference range establishment (pdf, 1 MB)
Britta A. Bettin, Bo Li, Kim Falkena, Ton G. van Leeuwen, Christian Gollwitzer, Zoltán Varga, Nadine Ajzenberg, Jovan P. Antovic, Pascale Berckmans, Edit I. Buzas, Randy P. Carney, Sean Cook, Françoise Dignat-George, Dorothee Faille, Bernd Giebel, Jennifer C. Jones, Yohan Kim, Romaric Lacroix, Joanne Lannigan, Fabrice Lucien, Katariina Maaninka, Erika G. Marques de Menezes, Annette Meyer, Rachel R. Mizenko, Inge Nelissen et al.
Journal of Extracellular Vesicles 14 (12), e70189 (2025)
269 A feasible machine learning framework for diagnosing stroke patients versus mimic conditions incorporating extracellular vesicle characterization and EHR features fusion (pdf, 2 MB)
Vasileios E. Katsigiannis, Ioannis Kakkos, Stavros Theofanis Miloulis, Ioannis A. Vezakis, Ourania Petropoulou, Joyce Rops, Naomi C. Buntsma, Edwin van der Pol, Dimitrios I. Fotiadis, and George K. Matsopoulos
Network Modeling Analysis in Health Informatics and Bioinformatics 15 (9), 1-3 (2025)
268 Extracellular vesicle release from immune cells in cutaneous Leishmaniasis: modulation by Leishmania (V.) braziliensis and reversal by antimonial therapy (pdf, 1 MB)
Vanessa Fernandes de Abreu Costa, Thaize Quiroga Chometon, Katherine Kelda Gomes de Castro, Melissa Silva Gonçalves Ponte, Maria Inês Fernandes Pimentel, Marcelo Rosandiski Lyra, Rienk Nieuwland, and Alvaro Luiz Bertho
Pathogens 14 (8), 771 (2025)
267 Identification of ischemic stroke patients based on plasma concentrations of extracellular vesicles (pdf, 1 MB)
Naomi C. Buntsma, Chi M. Hau, Mandy Los, Vivien M. Chen, Ton G. van Leeuwen, Yvo B.W.E.M. Roos, Rienk Nieuwland, Aleksandra Gasecka, and Edwin van der Pol
Translational Stroke Research 16, 2082- (2025)
266 Targeting oncofoetal chondroitin sulphate allows identification of tumour-derived extracellular vesicles (pdf, 3 MB)
Agustin Enciso-Martinez, Caroline Løppke, Joyce J. Koene, Jade Ebbelaar, Meike van der Geest, Mandy Los, Emma P.E.M. Boerrigter, Robert Dagil, Tobias Gustavsson, Elena E. Vidal-Calvo, Ton G. van Leeuwen, Roman I. Koning, Nick van Es, Ali Salanti, Edwin van der Pol, Rienk Nieuwland, Mette Ø. Ageræk, and Peter ten Dijke
Journal of Extracellular Vesicles 14 (6), e70106 (2025)
265 Monitoring and reporting the composition of plasma and serum to improve biobanks and comparability of extracellular vesicle research: Communication from the ISTH SSC Subcommittee on Vascular Biology (pdf, 1 MB)
Rienk Nieuwland, Fabrice Lucien, Dakota Gustafson, Metka Lenassi, Kimberly Martinod, and Yohei Hisada
Journal of Thrombosis and Haemostasis 23 (5), 1698-1703 (2025)
264 Endothelial cell-derived extracellular vesicles allow to differentiate between various endotypes of INOCA: a multicentre, prospective, cohort study (pdf, 2 MB)
Aleksandra Gąsecka, Piotr Szolc, Edwin van der Pol, Łukasz Niewiara, Bartłomiej Guzik, Paweł Kleczyński, Mariusz Tomaniak, Emilia Figura, Mateusz Zaremba, Marcin Grabowski, Janusz Kochman, Jacek Legutko, and Łukasz Kołtowski
Journal of Cardiovascular Translational Research 18 (2), 305-315 (2025)
263 Deciphering platelets: Are they cells or an evolved form of extracellular vesicles? (pdf, 1 MB)
Eric Boilard, Dylan Burger, Edit Buzas, Paolo Gresele, Kellie R. Muchlus, Nigel Mackman, Pia Siljander, and Rienk Nieuwland
Circulation Research 136 (4), 442-452 (2025)
262 Reproducibility of extracellular vesicle research (pdf, <1 MB)
Rossella Crescitelli, Juan Falcon-Perez, An Hendrix, Metka Lenassi, Le Thi Nguyet Minh, Takahiro Ochiya, Nicole Noren Hooten, Ursula Sandau, Clotilde Théry, and Rienk Nieuwland
Journal of Extracellular Vesicles 14 (1), e70036 (2025)

2024

261 TGF-beta regulates the release of breast cancer cell-derived extracellular vesicles and the sorting of their protein cargo by downregulating RAB27B expression (pdf, 3 MB)
Chao Li, Agustin Enciso-Martinez, Roman I. Koning, Mona Shahsavari, and Peter Ten Dijke
Journal of Extracellular Vesicles 13 (12), e70026 (2024)
260 Hemostatic conditions following autologous transfusion of fresh vs stored platelets in experimental endotoxemia: an open-label randomized controlled trial with healthy volunteers (pdf, 2 MB)
Stefan F. van Wonderen, Floor L.F. van Baarle, Anita M. Tuip-de Boer, Chantal A. Polet, Robin van Bruggen, Christie Vermeulen, Thomas R.L. Klei, Chi M. Hau, Rienk Nieuwland, Cornelis van 't Veer, Anna L. Peters, Sanne de Bruin, Alexander P.J. Vlaar, Bart J. Biemond, and Marcella C.A. Müller
Research and Practice in Thrombosis and Haemostasis 8 (8), 102612 (2024)
259 Size and fluorescence calibrated imaging flow cytometry: from arbitrary units to standard units (pdf, 1 MB)
Wouter W. Woud, Haley R. Pugsley, Britta A. Bettin, Zoltán Varga, and Edwin van der Pol
Cytometry Part A 105 (10), 752-762 (2024)
258 Saliva from persons with hemophilia A triggers coagulation via extrinsic tenase complexes (pdf, 2 MB)
Johannes Thaler, Carla Tripisciano, Daniel Kraemmer, Chi Hau, Nazanin Samadi, Wolfram Ruf, Ingrid Pabinger, Paul Knöbl, Rienk Nieuwland, and Cihan Ay
Blood 144 (25), 2666-2677 (2024)
257 Investigations on the hemostatic potential of physiological body fluids (pdf, 2 MB)
Johannes Thaler, Carla Tripisciano, and Rienk Nieuwland
Hamostaseologie 44 (8), 377-385 (2024)
256 MIBLood-EV: An Online Reporting Tool to Facilitate the Standardized Reporting of Preanalytical Variables and Quality Control of Plasma and Serum to Enhance Rigor and Reproducibility in Liquid Biopsy Research (pdf, <1 MB)
Dakota Gustafson, Rienk Nieuwland, and Fabrice Lucien
Biopreservation and Biobanking, in press.
255 Leukocyte extracellular vesicles predict progression of systolic dysfunction in heart failure with mildly reduced ejection fraction (LYCHEE) – a prospective, multicentre cohort study (external)
Aleksandra Gąsecka, Aleksander Siniarski, Piotr Duchnowski, Ewelina Błażejowska, Magdalena Gajewska, Kacper Karaban, Kinga Porębska, Aleksandra Reda, Sylwester Rogula, Bartosz Rolek, Dorota Słupik, Roksana Gozdowska, Marcin Kleibert, Dominika Zajkowska, Michał Grąt, Marcin Grabowski, Krzysztof J. Filipiak, Edwin van der Pol, and Rienk Nieuwland
Journal of Cardiovascular Translational Research 18 (1), 17-27 (2024)
254 Choice of size-exclusion chromatography column affects recovery, purity, and miRNA cargo analysis of extracellular vesicles from human plasma (pdf, 3 MB)
Jillian W.P. Bracht, Mandy Los, Edwin van der Pol, Sandra A.W.M. Verkuijlen, Monique A.J. van Eijndhoven, D. Michiel Pegtel, and Rienk Nieuwland
Extracellular Vesicles and Circulating Nucleic Acids 5 (3), 497-08 (2024)
253 Connecting through ISEV's developing social media landscape (pdf, <1 MB)
Tom A.P. Driedonks, Deborah C.I. Goberdhan, Sujata Mohanty, Sarah Williams, Rienk Nieuwland, Kenneth W. Witwer, and Ana Claudia Torrecilhas
Journal of Extracellular Vesicles 13 (7), e12475 (2024)
252 Extracellular vesicles to predict outcomes after transcatheter aortic valve implantation – a prospective, multicenter cohort study (pdf, 2 MB)
Radosław Wilimski, Jan Budzianowski, Michał Łomiak, Anna Olasińska‑Wiśniewska, Katarzyna Pieniak, Szymon Jędrzejczyk, Olaf Domaszk, Magdalena Chudzik, Krzysztof J. Filipiak, Jarosław Hiczkiewicz, Wojciech Faron, Tomasz Urbanowicz, Marek Jemielity, Marek Grygier, Marcin Grabowski, Mariusz Kuśmierczyk, Bartosz Rymuza, Zenon Huczek, Janusz Kochman, Edwin van der Pol, Rienk, Nieuwland, and Aleksandra Gąsecka
Journal of Cardiovascular Translational Research 17 (5), 992-1003 (2024)
251 Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology (pdf, 1 MB)
Amandine Bonifay, Nigel Mackmann, Yohei Hisada, Ana Teresa Azevedo Sachetto, Chi Hau, Elaine Gray, John Hogwood, Anat Aharon, Lina Badimon, Lucio Barile, Justine Baudar, Lennart Beckmann, Birke Benedikter, Sara Bolis, Tarik Bouriche, Marta Brambilla, Jacopo Burrello, Marina Camera, Elena Campello, Camille Ettelaie, Dorothée Faille, Sophie Featherby, Corentin Franco, Maite Guldenpfennig, John-Bjarne Hansen et al.
Journal of Thrombosis and Haemostasis 22 (10), 2910-2921 (2024)
250 Intradialytic cardiovascular injury is lowest in high-volume haemodiafiltration: a randomized cross-over trial in four intermittent dialysis strategies (pdf, 1 MB)
Peiyun Liu, Paul A. Rootjes, Camiel L.M. de Roij van Zuijdewijn, Chi M. Hau, Menso J. Nubé, Rienk Nieuwland, Gertrude Wijngaarden, and Muriel P.C. Grooteman
Clinical Kidney Journal 17 (6), sfae134 (2024)
249 M1-derived extracellular vesicles polarize recipient macrophages into M2-like macrophages and alter skeletal muscle homeostasis in a hyper-glucose environment (pdf, 8 MB)
Stefano Tacconi, Francesco Var, Carolina Sbarigia, Diana Vardanyan, Serena Longo, Francesco Mura, Federica Angilè, Audrey Jalabert, Ferninand Blangero, Assia Eljaafari, Laurence Canaple, Daniele Vergara, Francesco Paolo Fanizzi, Marco Rossi, Claire Crola Da Silva, Elizabeth Errazuriz‑Cerda, Christel Cassin, Rienk Nieuwland, Anna Maria Giudetti, Sophie Rome, and Luciana Dini
Cell Communication and Signaling 22 (193), 723 (2024)
248 Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches (pdf, 2 MB)
Joshua A. Welsh, Deborah C.I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A.P. Driedonks, Uta Erdbrügger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich et al.
Journal of Extracellular Vesicles 13 (2), e12404 (2024)
247 Poloxamer-188 as a wetting agent for microfluidic resistive pulse sensing measurements of extracellular vesicles (pdf, 2 MB)
Mona Shahsavari, Rienk Nieuwland, Ton G. van Leeuwen, and Edwin van der Pol
Plos One 19 (5), e0295849 (2024)
246 Lipoprotein apheresis affects the concentration of extracellular vesicles in patients with elevated lipoprotein (a) (pdf, 2 MB)
Joanna Marlęga-Linert, Aleksandra Gąsecka, Edwin van der Pol, Agnieszka Kuchta, Krzysztof J. Filipiak, Marcin Fijałkowski, Marcin Gruchała, Rienk Nieuwland, and Agnieszka Mickiewicz
Scientific Reports 14, 2762 (2024)
245 Proteome encoded determinants of protein sorting into extracellular vesicles (pdf, 2 MB)
Katharina Waury, Dea Gogishvili, Rienk Nieuwland, Madhurima Chatterjee, Charlotte E. Teunissen, and Sanne Abeln
Journal of Extracellular Biology 3 (1), e120 (2024)
244 Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies (pdf, 2 MB)
Ursula S. Sandau, Setty M. Magaña, Júlia Costa, John P. Nolan, Tsuneya Ikezu, Laura J. Vella, Hannah K. Jackson, Lissette Retana Moreira, Paola Loreto Palacio, Andrew F. Hill, Joseph F. Quinn, Kendall R. Van Keuren-Jensen, Trevor J. McFarland, Joanna Palade, Eric A. Sribnick, Huaqi Su, Kostas Vekrellis, Beth Coyle, You Yang, Juan M. Falcón-Perez, Rienk Nieuwland, Julie A. Saugstad, and nternational Society for Extracellular Vesicles Cerebrospinal Fluid Task Force
Journal of Extracellular Vesicles 13 (1), 12397 (2024)
243 A beginner's guide to study extracellular vesicles in human blood plasma and serum (pdf, 1 MB)
Rienk Nieuwland, and Pia R.M. Siljander
Journal of Extracellular Vesicles 13 (1), e12400 (2024)
242 Traceable characterization of hollow organosilica beads as potential reference materials for extracellular vesicle measurements with optical techniques (pdf, 3 MB)
Jérôme Deumer, Robin Schürmann, Anikó Gaál, Zoltán Varga, Britta Bettin, Edwin van der Pol, Rienk Nieuwland, David Ojeda, Aneta Sikora, Dorota Bartczak, Heidi Goenaga‑Infante, Johanna Noireaux, Mahrad Khakpour, Virpi Korpelainen, and Christian Gollwitzer
Discover Nano 19 (1), 14 (2024)

2023

241 Clinical applications and challenges in the field of extracellular vesicles (pdf, 11 MB)
Rienk Nieuwland, Agustin Enciso-Martinez, and Jillian W.P, Bracht
Medizinische Genetik 35 (4), 251-258 (2023)
240 MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research (pdf, <1 MB)
Fabrice Lucien, Dakota Gustafson, Metka Lenassi, Bo Li, Jacob J. Teske, Eric Boilard, on Hohenberg Katharina C. , alcón-Perez Juan M. , Alice Gualerzi, Antonia Reale, Jennifer C. Jones, Cecilia Lässer, Charlotte Lawson, Irina Nazarenko, Lorraine O'Driscoll, Ryan Pink, Pia R-M Siljander, Carolina Soekmadji, An Hendrix, Joshua A. Welsh, Kenneth W. Witwer, and Rienk Nieuwland
Journal of Extracellular Vesicles 12 (12), 12385 (2023)
239 MicroRNAs in extracellular vesicles released from epicardial adipose tissue promote arrhythmogenic conduction slowing (pdf, 3 MB)
Auriane C. Ernault, Rosan de Winter, Benedetta Fabrizi, Jillian WP Bracht, Chi Hau, Shirley CM van Amersfoorth, Eva R. Meulendijks, Anke J. Tijsen, Lucía Cócera Ortega, Ingeborg van der Made, Aleksandra Gasecka, Antoine H. Driessen, Rienk Nieuwland, Bastiaan J. Boukens, Edwin van der Pol, Joris R. de Groot, and Ruben Coronel
Heart Rhythm O2 4 (12), 805-14 (2023)
238 Anti-high-mobility group box-1 treatment strategies improve trauma-induced coagulopathy in a mouse model of trauma and shock (pdf, 1 MB)
Pieter H. Sloos, M. Adrie W. Maas, Joost C.M. Meijers, Rienk Nieuwland, Joris J.T.H. Roelofs, Nicole P. Juffermans, and Derek J.B. Kleinveld
British Journal of Anaesthesia 130 (6), 687-697 (2023)
237 Plasma extracellular vesicle test sample to standardize flow cytometry measurements (pdf, 1 MB)
Britta Bettin, Edwin van der Pol, and Rienk Nieuwland
Research and Practice in Thrombosis and Haemostasis 7 (4), 100181 (2023)
236 Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics (pdf, 4 MB)
Bert Dhondt, Cláudio Pinheiro, Edward Geeurickx, Joeri Tulkens, Glenn Vergauwen, Edwin van der Pol, Rienk Nieuwland, Anneleen Decock, Ilkka Miinalainen, Pekka Rappu, Jo Vandesompele, Pieter Mestdagh, Nicolaas Lumen, Olivier de Wever, and An Hendrix
Journal of Extracellular Vesicles 12 (5), 12315 (2023)
235 Preventing swarm detection in extracellular vesicle flow cytometry - a clinically applicable procedure (pdf, 2 MB)
Naomi C. Buntsma, Mona Shahsavari, Aleksandra Gąsecka, Rienk Nieuwland, Ton G. van Leeuwen, and Edwin van der Pol
Research and Practice in Thrombosis and Haemostasis 7 (4), 100171 (2023)
234 Standardization of extracellular vesicle concentration measurements by flow cytometry: the past, present and future (pdf, 1 MB)
Britta Bettin, Zoltán Varga, Rienk Nieuwland, and Edwin van der Pol
Journal of Thrombosis and Haemostasis 21 (8), 2032-44 (2023)
233 Tissue factor activity of small and large extracellular vesicles in different diseases (pdf, 1 MB)
Ana T.A. Sachetto, Sierra J. Archibald, Yohei Hisada, Axel Rosell, Sebastian Havervall, Nick van Es, Rienk Nieuwland, Robert A. Campbell, Elizabeth A. Middleton, Matthew T. Rondina, Charlotte Thålin, and Nigel Mackman
Research and Practice in Thrombosis and Haemostasis 7 (3), 100124 (2023)
232 Isolation-free measurement of single urinary extracellular vesicles by imaging flow cytometry (pdf, 3 MB)
Liang Wu, Wouter W. Woud, Carla C. Baan, Dennis A. Hesselink, Edwin van der Pol, Guido Jenster, and Karin Boer
Nanomedicine: Nanotechnology, Biology and Medicine 48, 102638 (2023)
231 A compendium of single extracellular vesicle flow cytometry (pdf, 4 MB)
Joshua A. Welsh, Ger J.A. Arkesteijn, Michel Bremer, Michael Cimorelli, Françoise Dignat-George, Bernd Giebel, André Görgens, An Hendrix, Martine Kuiper, Romaric Lacroix, Joanne Lannigan, Ton G. van Leeuwen, Estefanía Lozano-Andrés, Shoaib Rao, Stéphane Robert, Leonie de Rond, Vera A. Tang, Tobias Tertel, Xiaomei Yan, Marca H.M. Wauben, John Nolan, Jennifer C. Jones, Rienk Nieuwland, and Edwin van der Pol
Journal of Extracellular Vesicles 12 (2), 12299 (2023)
230 Platelet removal from human blood plasma improves detection of extracellular vesicle-associated miRNA (pdf, 1 MB)
Jillian W.P. Bracht, Mandy Los, Monique A.J. van Eijndhoven, Britta Bettin, Edwin van der Pol, D. Michiel Pegtel, and Rienk Nieuwland
Journal of Extracellular Vesicles 12 (2), 12302 (2023)
229 Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles (pdf, 6 MB)
Sean M. Davidson, Chantal M. Boulanger, Elena Aikawa, Lina Badimon, Lucio Barile, Christoph J. Binder, Alain Brisson, Edit Buzas, Costanza Emanueli, Felix Jansen, Miroslava Katsur, Romaric Lacroix, Sai Kiang Lim, Nigel Mackman, Manuel Mayr, Philippe Menasché, Rienk Nieuwland, Susmita Sahoo, Kaloyan Takov, Thomas Thum, Pieter Vader, Marca H.M. Wauben, Kenneth Witwer, and Joost P.G. Sluijter
Cardiovascular Research 119 (1), 45-63 (2023)

2022

228 Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry (pdf, 1 MB)
Angela A.F. Gankema, Bo Li, Rienk Nieuwland, and Edwin van der Pol
Cytometry Part A 101 (12), 1049-56 (2022)
227 Shock-driven endotheliopathy in trauma patients is associated with leucocyte derived extracellular vesicles (pdf, 1 MB)
Romein, W.G. Dujardin, Jeske E.C. Kisters, Mathijs R. Wirtz, Najat Hajji, Anita M. Tuip-de Boer, Jakob Stensballe, Pär I. Johansson, Karim Brohi, Ross A. Davenport, Christine Gaarder, Simon Stanworth, Marc Maegele, Rienk Nieuwland, Edwin van der Pol, and Nicole P. Juffermans
International Journal of Molecular Sciences 23 (24), 15990 (2022)