Methods and procedures developed by Amsterdam Vesicle Center

Methods and procedures developed by us

Deriving extracellular vesicle size from scatter intensities measured by flow cytometry (pdf, <1 MB)
Leonie de Rond, Frank A.W. Coumans, Rienk Nieuwland et al.
Current Protocols in Cytometry 86 (1), e43 (2018)

Single-step isolation of extracellular vesicles by size-exclusion chromatography (pdf, 2 MB)
Anita N. Böing, Edwin van der Pol, Anita E. Grootemaat et al.
Journal of Extracellular Vesicles 3, 23430 (2014)

Protocol for measuring concentrations of extracellular vesicles in human blood plasma with flow cytometry (pdf, 1 MB)
Najat Hajji, Chi M. Hau, Rienk Nieuwland, and Edwin van der Pol
In: Maurizio Federico and Barbara Ridolfi, ed. Extracellular vesicles in diagnosis and therapy, 1^st edn., New York: Springer Nature, 2022: 55-75

A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing (pdf, 2 MB)
Robert Vogel, Frank A.W. Coumans, Raluca G. Maltesen et al.
Journal of Extracellular Vesicles 5 (1), 31242 (2016)

ACS	de Rond, L.; Coumans, F. A. W.; Nieuwland, R.; van Leeuwen, T. G.; van der Pol, E. Deriving extracellular vesicle size from scatter intensities measured by flow cytometry. Curr. Protoc. Cytom. 2018, 86 (1), e43.
AMA	de Rond L, Coumans FAW, Nieuwland R, van Leeuwen TG, van der Pol E. Deriving extracellular vesicle size from scatter intensities measured by flow cytometry. . 2018;86(1):e43.
DOI	10.1002/cpcy.43
IEEE	L. de Rond, F. A. W. Coumans, R. Nieuwland, T. G. van Leeuwen, and E. van der Pol, “Deriving extracellular vesicle size from scatter intensities measured by flow cytometry,” Curr. Protoc. Cytom., vol. 86, no. 1, pp. e43, Aug. 2018.
NLM	de Rond L, Coumans FAW, Nieuwland R, van Leeuwen TG, van der Pol E. Deriving extracellular vesicle size from scatter intensities measured by flow cytometry. . 2018 Aug;86(1):e43.

ACS	Böing, A. N.; van der Pol, E.; Grootemaat, A. E.; Coumans, F. A. W.; Sturk, A.; Nieuwland, R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J. Extracell. Vesicles 2014, 3, 23430.
AMA	Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. . 2014;3:23430.
DOI	10.3402/jev.v3.23430
IEEE	A. N. Böing, E. van der Pol, A. E. Grootemaat, F. A. W. Coumans, A. Sturk, and R. Nieuwland, “Single-step isolation of extracellular vesicles by size-exclusion chromatography,” J. Extracell. Vesicles, vol. 3, pp. 23430, Sep. 2014.
NLM	Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. . 2014 Sep;3:23430.

ACS	Cimorelli, M.; Nieuwland, R.; Varga, Z.; van der Pol, E. Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing. PLoS One 2021, 16 (4), e0249603.
AMA	Cimorelli M, Nieuwland R, Varga Z, van der Pol E. Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing. . 2021;16(4):e0249603.
DOI	10.1371/journal.pone.0249603
IEEE	M. Cimorelli, R. Nieuwland, Z. Varga, and E. van der Pol, “Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing,” PLoS One, vol. 16, no. 4, pp. e0249603, Apr. 2021.
NLM	Cimorelli M, Nieuwland R, Varga Z, van der Pol E. Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing. . 2021 Apr;16(4):e0249603.

ACS	Vogel, R.; Coumans, F. A. W.; Maltesen, R. G.; Böing, A. N.; Bonnington, K. E.; Broekman, M. L.; Broom, M. F.; Buzás, E. I.; Christiansen, G.; Hajji, N.; et al. A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing. J. Extracell. Vesicles 2016, 5 (1), 31242.
AMA	Vogel R, Coumans FAW, Maltesen RG, et al. A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing. . 2016;5(1):31242.
DOI	10.3402/jev.v5.31242
IEEE	R. Vogel et al., “A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing,” J. Extracell. Vesicles, vol. 5, no. 1, pp. 31242, Sep. 2016.
NLM	Vogel R, Coumans FAW, Maltesen RG, Böing AN, Bonnington KE, Broekman ML, et al. A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing. . 2016 Sep;5(1):31242.