Frameworks and guidelines by Amsterdam Vesicle Center

Frameworks and guidelines lead by us

A beginner's guide to study extracellular vesicles in human blood plasma and serum (pdf, 1 MB)
Rienk Nieuwland, and Pia R.M. Siljander
Journal of Extracellular Vesicles 13 (1), e12400 (2024)

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ACS	Nieuwland, R.; Siljander, P. R. A beginner's guide to study extracellular vesicles in human blood plasma and serum. J. Extracell. Vesicles 2024, 13 (1), e12400.
AMA	Nieuwland R, Siljander PR. A beginner's guide to study extracellular vesicles in human blood plasma and serum. J Extracell Vesicles. 2024;13(1):e12400.
DOI	10.1002/jev2.12400
IEEE	R. Nieuwland, and P. R. Siljander, “A beginner's guide to study extracellular vesicles in human blood plasma and serum,” J. Extracell. Vesicles, vol. 13, no. 1, pp. e12400, Jan. 2024.
NLM	Nieuwland R, Siljander PR. A beginner's guide to study extracellular vesicles in human blood plasma and serum. J Extracell Vesicles. 2024 Jan;13(1):e12400.

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Methodological guidelines to study extracellular vesicles (pdf, 1 MB)
Frank A.W. Coumans, Alain R. Brisson, Edit I. Buzas et al.
Circulation Research 120 (10), 1632-48 (2017)

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ACS	Coumans, F. A. W.; Brisson, A. R.; Buzas, E. I.; Dignat-George, F.; Drees, E. E. E.; El-Andaloussi, S.; Emanueli, C.; Gasecka, A.; Hendrix, A.; Hill, A. F.; et al. Methodological guidelines to study extracellular vesicles. Circ. Res. 2017, 120 (10), 1632-1648.
AMA	Coumans FAW, Brisson AR, Buzas EI, et al. Methodological guidelines to study extracellular vesicles. Circ Res. 2017;120(10):1632-1648.
DOI	10.1161/CIRCRESAHA.117.309417
IEEE	F. A. W. Coumans et al., “Methodological guidelines to study extracellular vesicles,” Circ. Res., vol. 120, no. 10, pp. 1632-1648, May. 2017.
NLM	Coumans FAW, Brisson AR, Buzas EI, Dignat-George F, Drees EEE, El-Andaloussi S, et al. Methodological guidelines to study extracellular vesicles. Circ Res. 2017 May;120(10):1632-48.

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MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research (pdf, <1 MB)
Fabrice Lucien, Dakota Gustafson, Metka Lenassi et al.
Journal of Extracellular Vesicles 12 (12), 12385 (2023)

Supplements Journal of Extracellular Vesicles 12 (12), 12385 (2023)

Suppl. 1: Supplementary Material (pdf, <1 MB)

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ACS	Lucien, F.; Gustafson, D.; Lenassi, M.; Li, B.; Teske, J. J.; Boilard, E.; , o. H. K. C.; , a. J. M.; Gualerzi, A.; Reale, A.; et al. MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. J. Extracell. Vesicles 2023, 12 (12), 12385.
AMA	Lucien F, Gustafson D, Lenassi M, et al. MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. J Extracell Vesicles. 2023;12(12):12385.
DOI	10.1002/jev2.12385
IEEE	F. Lucien et al., “MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research,” J. Extracell. Vesicles, vol. 12, no. 12, pp. 12385, Dec. 2023.
NLM	Lucien F, Gustafson D, Lenassi M, Li B, Teske JJ, Boilard E, et al. MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. J Extracell Vesicles. 2023 Dec;12(12):12385.

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MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments (pdf, 3 MB)
Joshua A. Welsh, Edwin van der Pol, Ger J.A. Arkesteijn et al.
Journal of Extracellular Vesicles 9, 1713526 (2020)

Supplements Journal of Extracellular Vesicles 9, 1713526 (2020)

Suppl. 1: Supplemental information (xlsx, <1 MB)

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ACS	Welsh, J. A.; van der Pol, E.; Arkesteijn, G. J. A.; Bremer, M.; Brisson, A.; Coumans, F.; Dignat-George, F.; Duggan, E.; Ghiran, I.; Giebel, B.; et al. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. J. Extracell. Vesicles 2020, 9, 1713526.
AMA	Welsh JA, van der Pol E, Arkesteijn GJA, et al. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. J Extracell Vesicles. 2020;9:1713526.
DOI	10.1080/20013078.2020.1713526
IEEE	J. A. Welsh et al., “MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments,” J. Extracell. Vesicles, vol. 9, pp. 1713526, Feb. 2020.
NLM	Welsh JA, van der Pol E, Arkesteijn GJA, Bremer M, Brisson A, Coumans F, et al. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. J Extracell Vesicles. 2020 Feb;9:1713526.

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Frameworks and guidelines to which we contributed

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) (pdf, 44 MB)
Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch et al.
European Journal of Immunology 49 (10), 1457-973 (2019)

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ACS	Cossarizza, A.; Chang, H.; Radbruch, A.; Acs, A.; Adam, D.; Adam-Klages, S.; Agace, W. W.; Aghaeepour, N.; Akdis, M.; Allez, M.; et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur. J. Immunol. 2019, 49 (10), 1457-1973.
AMA	Cossarizza A, Chang H, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol. 2019;49(10):1457-1973.
DOI	10.1002/eji.201970107
IEEE	A. Cossarizza et al., “Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition),” Eur. J. Immunol., vol. 49, no. 10, pp. 1457-1973, Oct.. 2019.
NLM	Cossarizza A, Chang H, Radbruch A, Acs A, Adam D, Adam-Klages S, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol. 2019 Oct.;49(10):1457-973.

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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches (pdf, 2 MB)
Joshua A. Welsh, Deborah C.I. Goberdhan, Lorraine O'Driscoll et al.
Journal of Extracellular Vesicles 13 (2), e12404 (2024)

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ACS	Welsh, J. A.; Goberdhan, D. C. I.; O'Driscoll, L.; Buzas, E. I.; Blenkiron, C.; Bussolati, B.; Cai, H.; Di Vizio, D.; Driedonks, T. A. P.; Erdbrügger, U.; et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J. Extracell. Vesicles 2024, 13 (2), e12404.
AMA	Welsh JA, Goberdhan DCI, O'Driscoll L, et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024;13(2):e12404.
DOI	10.1002/jev2.12404
IEEE	J. A. Welsh et al., “Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches,” J. Extracell. Vesicles, vol. 13, no. 2, pp. e12404, Feb. 2024.
NLM	Welsh JA, Goberdhan DCI, O'Driscoll L, Buzas EI, Blenkiron C, Bussolati B, et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024 Feb;13(2):e12404.

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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines (pdf, 1 MB)
Clotilde Théry, Kenneth W. Witwer, Elena Aikawa et al.
Journal of Extracellular Vesicles 7 (1), 1535750 (2018)

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ACS	Théry, C.; Witwer, K. W.; Aikawa, E.; Alcaraz, M. J.; Anderson, J. D.; Andriantsitohaina, R.; Antoniou, A.; Arab, T.; Archer, F.; Atkin-Smith, G. K.; et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J. Extracell. Vesicles 2018, 7 (1), 1535750.
AMA	Théry C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018;7(1):1535750.
DOI	10.1080/20013078.2018.1535750
IEEE	C. Théry et al., “Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines,” J. Extracell. Vesicles, vol. 7, no. 1, pp. 1535750, Sep. 2018.
NLM	Théry C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, Andriantsitohaina R, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018 Sep;7(1):1535750.

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